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Description: | CRICETULUS GRISEUS No 738 TESTIS WITH LEISH. DONOVANI 20-5-43 Zenker (printed on a label gummed to the glass slde). Feulgen (written on the paper label). 738 B Feulgen (scratched on the glass slide). MANU PONTERCORVO,Guido. May 20, 1943 Glasgow University, Glasgow, Scotland. This is a specimen of the testes of *Cricetulus griseus barabensis*, the Chinese striped hamster fixed with Feulgen's stain, A specific histochemical test for chromosonal material or DNA using sections or cells that are hydrolysed in hydrochloric acid producing apurinic acid (DNA, from which the purine bases have been removed) then reacted with Schiff's reagent to produce a magenta stain. Robert Feulgen (1884-1955) German nucleic acid biochemist and cytochemist, and H. Rossenbeck, in 1924.
The specimen appears to have also been treated with Zenker's solution. A stock solution consists of mercuric chloride, potassium bichromate and sodium sulphate. The working solution consists of 95 % Zenker stock + 5 % glacial acetic acid. When glacial acetic acid has been replaced by formalin, it is called formol-Zenker fixative. Freidrich Albert von Zenker (1825-1898) German physician and pathologist.
Schiff's reagent. An aqueous solution of basic fuchsin or pararosaniline, 4,4'-[(4-imino-2,5-cyclohexadien-1-ylidene)methylene]bisbenzenamine monohydrochloride, Red number 9. Hugo Schiff (1834-1915) German chemist.
The process undergone was meiosis; the cell division in sexually reproducing organisms causing a reduction in the number of chromosomes, in the reproductive cells, from diploid (paired chromosomes) to haploid (single chromosomes) thus leading to the production of gametes (asexual) animals or plants.
The specimen was obtained from an animal killed by infection with *Leishmania donovani (infantum)* parasitic hemoflagellate bacterium of the subgenus *Leishmania leishmania* that infects man and animals and causes visceral *leishmaniasis*; the sandfly genera Phlebotomus and Lutzomyia are the normal vectors."
Appears to be connected with work done on Chinese sandflies as vectors for *Leishmania* published in 1931 by professor Edward Hindle (1886-1973) Chair of Zoology at Glasgow University. | License: | http://www.hmag.gla.ac.uk/spirit/rights/ | Publisher: | Hunterian Museum and Art Gallery, University of Glasgow | Rights holder: | Hunterian Museum and Art Gallery, University of Glasgow | Subjects: | SCIENTIFIC COLLECTION : | Source: | Hunterian Museum | Creator: | Hunterian Museum and Art Gallery, University of Glasgow | Identifier: | http://www.huntsearch.gla.ac.uk/cgi-bin/... | Language: | en-GB | Go to resource |
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