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Description: | CRICETULUS GRISEUS No T4 TESTIS WITH LEISH. TROPICA 20-5-43 REGAUD FEULGEN SEMMENS. (printed on a label gummed to the glass slde). BOUIN (written on the paper label). T4 Ring Feulg Semmens (scratched on the glass slide). MANU PONTERCORVO,Guido. May 20, 1943 Glasgow University, Glasgow, Scotland. This is a specimen of the testes of *Cricetulus griseus barabensis*, the Chinese striped hamster treated with Regaud's stain, which is a modified Heidenhain's stain by the addition of glycerol in the mixture. This gives a more uniform stain. Both methods depend on mordanting (fixing) in fresh 5% aqueous iron alum followed by the addition of hematoxylin (Natural Black 1, derived from the logwood tree Hematoxylon campechianum) and then differentiation in fresh 5% aqueous iron alum. The stain, 1 gm hematoxylin in a solution of 10 ml. 90% ethanol in 80 ml. distilled water with10 m of glycerin, is then added. Claude Regaud (1870-1941) French radiologist.
Schiff's reagent. An aqueous solution of basic fuchsin or pararosaniline, 4,4'-[(4-imino-2,5-cyclohexadien-1-ylidene)methylene]bisbenzenamine monohydrochloride, Red number 9. Hugo Schiff (1834-1915) German chemist.
The specimen also appears to have been treated with Semmen's stain, an addition to Feulgen's procedure that includes soaking the specimen in 5% sodium carbonate for an hour. C.S. Semmens and P.N. Bhaduri, 1940
The specimen appears to have also been treated with Feulgen's stain, A specific histochemical test for chromosonal material or DNA using sections or cells that are hydrolysed in hydrochloric acid producing apurinic acid (DNA, from which the purine bases have been removed) then reacted with Schiff's reagent to produce a magenta stain. Robert Feulgen (1884-1955) German nucleic acid biochemist and cytochemist, and H. Rossenbeck, in 1924.
According to the information scratched on the glass the specimen had also be treated with mammalian Ringer's solution. A solution of recently boiled distilled water containing 8.6g sodium chloride, 0.3g potassium chloride, and 0.033 g calcium chloride per litre, the same concentrations as their occurrence in body fluids. Sydney Ringer (1834-1910) British clinician and pharmacologist.
The process undergone was meiosis; the cell division in sexually reproducing organisms causing a reduction in the number of chromosomes, in the reproductive cells, from diploid (paired chromosomes) to haploid (single chromosomes) thus leading to the production of gametes (asexual) animals or plants.
The specimen was obtained from an animal killed by infection with *Leishmania tropica* parasitic hemoflagellate bacterium of the subgenus *Leishmania leishmania* that infects man and animals and causes visceral *leishmaniasis*; the sandfly genera Phlebotomus and Lutzomyia are the normal vectors."
Appears to be connected with work done on Chinese sandflies as vectors for *Leishmania* published in 1931 by professor Edward Hindle (1886-1973) Chair of Zoology at Glasgow University. | License: | http://www.hmag.gla.ac.uk/spirit/rights/ | Publisher: | Hunterian Museum and Art Gallery, University of Glasgow | Rights holder: | Hunterian Museum and Art Gallery, University of Glasgow | Subjects: | SCIENTIFIC COLLECTION : | Source: | Hunterian Museum | Creator: | Hunterian Museum and Art Gallery, University of Glasgow | Identifier: | http://www.huntsearch.gla.ac.uk/cgi-bin/... | Language: | en-GB | Go to resource |
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