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Description: | CRICETULUS GRISEUS No T4 TESTIS WITH LEISH. TROPICA 20-5-43. (printed on a label gummed to the glass slde). T4 Reagent Ehrlich (scratched on the glass slide). Regaud Ehrlich Haem + Gent. Violet (written in ink on the label). MANU PONTERCORVO,Guido. May 20, 1943 Glasgow University, Glasgow, Scotland. This is a specimen of the testes of *Cricetulus griseus barabensis*, the Chinese striped hamster treated with Regaud's stain. A modified Heidenhain's stain by the addition of glycerol in the mixture, which gives a more uniform stain. Both methods depend on mordanting in fresh 5% aqueous iron alum followed by the addition of hematoxylin (Natural Black 1, derived from the logwood tree Hematoxylon campechianum) and then differentiation in fresh 5% aqueous iron alum. The stain, 1 gm hematoxylin in a solution of 10 ml. 90% ethanol in 80 ml. distilled water with10 m of glycerin, is then added. Claude Regaud (1870-1941) French radiologist.
Rudolph Peter Heinrich Heidenhain (1834-1897) German physiologist.
It has also been treated with Ehrlich's stain. Methylene blue or 3,7-bis(dimethylamino)-phenazathionium chloride tetramethylthionine chloride.Paul Ehrlich (1854-1915) German physician and biochemist. First described as a biological stain in1886.
Two colourants have also been added. Haem, which is an organic structure known as a porphorin, also known as a tetrapyrrole ring structure, to which iron is bound. It forms an iron-organic complex that is an oxygen scavenging red pigment, which is part of peroxidase a constituent of the haemoglobin in red blood cells. Presumably this was added to see if it formed a polymerase as in cases of malaria.
Also added was gentian violet violet. Hans Christian Joachim Gram (1853-1938) Danish botanist and bacteriologist who in about 1884 discovered that a dye derived from coal-tar, hexamethylpararosaniline chloride or gentian violet, could be used to stain certain kinds of bacteria, Gram positive, a blue violet colour and the others, Gram negative, pink.
The process undergone was meiosis; the cell division in sexually reproducing organisms causing a reduction in the number of chromosomes, in the reproductive cells, from diploid (paired chromosomes) to haploid (single chromosomes) thus leading to the production of gametes (asexual) animals or plants.
The specimen was obtained from an animal killed by infection with *Leishmania tropica* parasitic hemoflagellate bacterium of the subgenus *Leishmania leishmania* that infects man and animals and causes visceral *leishmaniasis*; the sandfly genera Phlebotomus and Lutzomyia are the normal vectors."
Appears to be connected with work done on Chinese sandflies as vectors for *Leishmania* published in 1931 by professor Edward Hindle (1886-1973) Chair of Zoology at Glasgow University. | License: | http://www.hmag.gla.ac.uk/spirit/rights/ | Publisher: | Hunterian Museum and Art Gallery, University of Glasgow | Rights holder: | Hunterian Museum and Art Gallery, University of Glasgow | Subjects: | SCIENTIFIC COLLECTION : | Source: | Hunterian Museum | Creator: | Hunterian Museum and Art Gallery, University of Glasgow | Identifier: | http://www.huntsearch.gla.ac.uk/cgi-bin/... | Language: | en-GB | Go to resource |
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